Triglycerides,and

A sensitive (5 to 100g) and reproducible (±3.54 % SD) method is described for determining monoglycerides, diglycerides, triglycerides, and free fatty acids. After these lipid classes are separated from extracts of plasma or tissues by a single thin.layer chromatographic development, they are eluted into concentrated H2S04at 200#{176}C.The resulting color is stable for more than six days. Additional Keyphrases thin-layer chromatography #{149}elution from TLC with Ff5804 IPublished methods for the determination of plasma triglycerides are of two kinds, enzymatic (1-3) and colorimetric (4-7). Both require prelimi-nary removal of phospholipids and saponification of triglycerides. With the enzymatic method, glycerin released is quantitatively determined with the specific enzyme GPDH ’ and NAD. In the colorimetric methods, glycerin is oxidized to formaldehyde with periodic acid. The formaldehyde is then reacted to form a chromotrope with chromotropic acid (4, 5, 7) or acetylacetone (6). FFA are extracted from the plasma and deter-mined acidimetrically as described by Dole (8) and others (9-11). The colorimetric determination of plasma FFA as described by Duncombe (12) is not an alternative procedure to the Dole method, since, as Duncombe himself admits, phospholipids inter-fere with this determination. Measurement in plasma of both triglycerides and FFA requires a long and laborious standardiza-tion procedure to obtain good reproducibility. Further difilculties are encountered when attempts are made to determine glycerides and FFA quanti-tatively in liver-problems mainly resulting from the presence of monoglycerides and diglyceride