The action of two restriction endonucleases on polyoma virus DNA has been examined and the sites at which they cleave the DNA located. One ofthe enzymes, KpnI from Klebsiella pneumoniae OK8, cleaves polyoma DNA twice at about I 1.6 and 59"2 ~ from the EcoRI site. The other enzyme, PstI from Providencia stuartii 164, cleaves polyoma DNA five times at about 14"8, 16"5, 32"6, 5o'3 and 8o.o ~ from the EcoRI site. Some of the cleavages produced by these enzymes alone, or in conjunction with other endonucleases, may be of use in the isolation of regions of particular interest from the virus DNA
The primary action of restriction endonuclease, cleaving infecting DNA, has been demonstrated in viv...
Type II restriction endonucleases are components of restriction modification systems that protect ba...
Many types of restriction enzymes cleave DNA away from their recognition site. Using the type III re...
The Hemophilus influenzae HindII endonuclease cleaves polyoma DNA into two fragments, approximately ...
Ph.D.BiochemistryUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue...
Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequen-ce 5'--G-G-N-C-C—3...
The diversity of reaction mechanisms employed by Type II restriction enzymes was investigated by ana...
Spontaneous mutants of polyoma virus have been isolated which lack either one or both of the two end...
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolate...
The 29 sites in the polyoma genome cleaved by endonuclease (endo) Alul and the site cleaved by endo ...
The kinetic data obtained from examination of restriction enzyme fragments give a direct determinati...
Digestion of DNA with restriction endonucleases is the first step in many gene manipulation projects...
Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, r...
A restriction endonuclease, SalI, has been partially purified from Streptomyces albus G. This enzyme...
AbstractMost restriction endonucleases bridge two target sites before cleaving DNA: examples include...
The primary action of restriction endonuclease, cleaving infecting DNA, has been demonstrated in viv...
Type II restriction endonucleases are components of restriction modification systems that protect ba...
Many types of restriction enzymes cleave DNA away from their recognition site. Using the type III re...
The Hemophilus influenzae HindII endonuclease cleaves polyoma DNA into two fragments, approximately ...
Ph.D.BiochemistryUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue...
Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequen-ce 5'--G-G-N-C-C—3...
The diversity of reaction mechanisms employed by Type II restriction enzymes was investigated by ana...
Spontaneous mutants of polyoma virus have been isolated which lack either one or both of the two end...
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolate...
The 29 sites in the polyoma genome cleaved by endonuclease (endo) Alul and the site cleaved by endo ...
The kinetic data obtained from examination of restriction enzyme fragments give a direct determinati...
Digestion of DNA with restriction endonucleases is the first step in many gene manipulation projects...
Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, r...
A restriction endonuclease, SalI, has been partially purified from Streptomyces albus G. This enzyme...
AbstractMost restriction endonucleases bridge two target sites before cleaving DNA: examples include...
The primary action of restriction endonuclease, cleaving infecting DNA, has been demonstrated in viv...
Type II restriction endonucleases are components of restriction modification systems that protect ba...
Many types of restriction enzymes cleave DNA away from their recognition site. Using the type III re...