Short communications SPONTANEOUS GYNOGENESIS IN IDE, LEUCISCUS IDUS (L.)

ABSTRACT. The aim of the study was to obtain information regarding the spontaneous formation of gynogens in ide, Leuciscus idus (L.). Oocytes obtained from orfe and activated with the milt of dace, Leuciscus leuciscus (L.), were used for the study. Several control groups were established to determine the quality of gametes and the quality of the diluted semen. Ploidy in the offspring was determined by analyzing the number of nucleoli in the cells. Spontaneous diploid individuals appeared in groups in which the biological quality of the oocytes was poor. The percentage of these individuals was not large, and the average was a maximum of 0.4%. Key words: GYNOGENS, GENOME MANIPULATION, BIOTECHNOLOGY One of the methods of obtaining fish with certain desirable characters is gynogenesis (Chourrout et al. 1980), through which offspring are produced with mater-nal genetic material. This is a two-step process. First, the genetic material in the sperm is destroyed with X, ã, or UV irradiation (Varadaraj 1993). The resulting sperm is only able to activate eggs. The activated egg begins to develop into a haploid zygote. If the embryo is to develop normally, it must be subjected to an environmental shock, either thermal, chemical, or pressure (Komen et al. 1988). There are two moments durin