Running Title: Autoreactivity vs pathogenicity in TCR Rg mice Corresponding Author:

Objective:Type 1 diabetes is mediated by T cell entry into pancreatic islets and destruction of insulin-producing β cells. The relative contribution of T cells specific for different autoantigens is largely unknown as relatively few have been assessed in vivo. Research Design and Methods: We generated mice possessing a monoclonal population of T cells expressing one of 17 T cell receptors (TCR) specific for either known autoantigens (GAD65, IA2, IA2β/phogrin and insulin), unknown islet antigens or control antigens on a NOD.scid background using retroviral-mediated stem cell gene transfer and 2A-linked multicistronic retroviral vector