Supplemental Experimental Procedures

For mutant alleles tested for aldicarb sensitivity see Table S1. Alleles used for aldicarb resistant mutant backgrounds (Fig. 2 and S1) are as follows: unc-17(e113), rab-3(js49), snb-1(md247), egl-30(ad806), egl-8(sa47), egl-10(n692), unc-31(e298), egl-3(nr2090), egl-21(n476), and gta-1(ok517). Allele lesions can be found at www.wormbase.org. The strains used for quantitative studies of SNB-1 fluorescence in the GABAergic and cholinergic DA neurons carried the integrants juIs1 (Punc-25::SNB-1::GFP) and nuIs152 (Punc129::GFP::SNB-1) respectively. The strains used for analysis of soluble GFP in the GABAergic and cholinergic motor neurons carried the integrants oxIs12 (Punc-47::GFP) and nuIs208 (Punc129::GFP). The GFP-tagged GABAAR integrant is oxIs22 (Bamber et al., 1999). The transgene nuIs283, which expresses both RFP-tagged RAB-3 (with the unc-25 promoter) and GFP-tagged GABAAR (with the myo-3 promoter), was used to analyze the alignment of pre- and post-synaptic elements at GABAergic NMJs. RNAi Feeding Experiments and Screen For the primary screen, bacteria containing each RNAi clone were cultured in 400 µl LB media containing 50 µg/ml Ampicillin for 10-12 hrs. 40 µl of each culture was spotted in a single well of a 24-well plate containing NGM agar, 6 mM IPTG and 25 µg/ml Carbenicillin. After overnight incubation at room temperature, 100 eggs were plated per well and incubated at 20°C for 3 days. After 3 days of growth, 25 young adults per well were transferred into NG