COLD ADAPTED AMYLASE AND PROTEASE FROM NEW STREPTOMYCES 4ALGA ANTARCTIC STRAIN

The ability of a new polar strain coded Streptomyces 4Alga, isolated from vegetation samples from East Antarctica, to biosynthesis cold adapted amylases and proteases was investigated. Thermal inactivation studies shown that alpha-amylase enzyme retained almost 90 % and 80 % of its activity at optimum temperature (30°C) during the interval of 20-60 minutes of incubation. Instead, after 20 minutes of incubation at low temperature (20°C) alpha-amylase activity decreased. At optimum temperature beta-amylase retained almost 80 % of its activity after 50 min of incubation. At 20°C beta-amylase showed 60 % relative activity after 60 min of incubation. While, protease retained approximately 75 % of its activity at optimum temperature, at lower temperature seamed to be less stable after one hour of incubation. The reported enzymes may have wide spread application for detergent and pharmaceutical and food industry