1EFFECTIVENESS OF RECOMBINANT FIBROBLAST GROWTH FACTOR The Effectiveness of Recombinant Fibroblast Growth Factor in Promoting Growth in Non-

Cells require growth factors in the process of growing and dividing. When culturing cells in the laboratory growth factors are sometimes added in the form of fetal bovine serum(FBS) to promote growth and division. This project will determine if recombinant fibroblast growth factor(FGF) produced in E.coli can be used to stimu-late growth and division of cells in tissue culture. The main emphasis of this experiment is to transfer the DNA needed to produce FGF to E.coli and purify it out of solution in an active form. The project has determined that FGF produced in E.coli can be purified out of solution in an active form. The project also demonstrat-ed the effectiveness of FGF at stimulating growth when compared to other agents that stimulated growth