INCORPORATION OF A WINTER BARLEY CHROMOSOME SEGMENT INTO CULTIVATED WHEAT AND ITS CHARACTERIZATION WITH GISH, FISH AND SSR MARKERS

Abstract: Spontaneous wheat-barley translocation chromosomes were detected by genomic in situ hybridisation (GISH) in the progenies of wheat/barley addition lines produced from the wheat cultivar Asakaze komugi and the Ukrainan six-rowed barley cultivar Manas. The homozygous translocation line was selected from a heterozygous translocation line with the help of molecular markers and fluorescence in situ hybridisation (FISH) using repetitive DNA probes. The wheat chromosome arm involved in the translocation was identified by FISH as 4BS. The barley chromosome segment could not be unequivocally determined with the hybridisation of the repetitive DNA sequences used in the present work (Afa family, pSc119.2, pTa71). The microsatellite marker analysis revealed the presence of an almost complete 7HL chromosome arm, but the centromeric region of 7HL was missing from this translocation line. The rearranged chromosome, identified as 4BS.7HL with a centromeric deletion of 7HL, represents a unique genetic material which can be used for the physical mapping of genes or genetic markers within 7HL. As the barley chromosome 7H is considered to be the most important chromosome for drought tolerance, the translocation line will make it possible to reveal the effect of the abiotic stress-related genes situated on the incorporated 7HL segment in the genetic background of wheat