Lecturer Department of Pharmacology

Objective: To test in vitro combination of fusidic acid and amikacin against infections caused by methicillin resistant Staphlococus aureus (MRSA). Methodology: In vitro study conducted in Department of Pharmacology and Microbiology, Dr. Ziauddin Medical University. The duration of study was March 2004- February 2005. Antibiotic susceptibility testing was done by Kirby Bauer’s disc diffusion method and by minimum inhibitory concentration (MIC) by broth macrodilution and checkerboard technique for synergy. FIC (frac-tional inhibitory concentrations) were calculated. Results: MIC of fusidic acid was 0.03-1µg/ml and amikacin 0.5-16µg/ml respectively. The combination of these demonstrated synergy. Evidence of synergy correlated directly with the MICs of fusidic acid and amikacin. Conclusion: Combination therapy with fusidic acid and amikacin may be a reasonable alternative in the treatment of infections caused by MRSA isolates and encourages clinical evaluation