SNP and Small Indel Discovery with SOLiD ™ System Sequence Reads Using NextGENe ™ Software and Condensation Tool™

Single Nucleotide Polymorphism (SNP) discovery and screening are important for disease discovery and treatment, such as asthma, addictions and cancer (1), as well as association studies (2). Some of the more mature methods for SNP detection include techniques such as Sanger sequencing, the many types of heteroduplex analysis (3), mass spectrometry (4) and microarrays (5). The Applied Biosystems SOLiD System employs the sequencing by ligation technique to generate several gigabases of short sequenc