In vitro Regeneration of Impatiens campanulata Wight an Important Grass Land Plant

Abstract: Long term in vitro cultures of plant tissue spontaneously change at morphological, cellular and biochemical level. Impatiens campanulata tissues were cultivated formore than 70 years. Leaf explants excised from plantlets propagated in vitro. Subcultured on hormone-free MS medium, transformed tissue continued to grow in an unorganized way forming malformed shoots which never rooted. The aim of this study was to establish the Optimization of nutrient media, plant growth regulators, carbon source antioxidants and additives for achieving enhanced axillary budding from stem explants of Impatiens campanulata. Key words: Impatiens campanulata Nutrient media Axillary’s bud MS medium INTRODUCTION Indian system of medicine chiefly depends on Man had a long struggle to achieve mastery over been abundantly available in many parts of the Indian powerful force for nature, he has always turned to plants peninsula since time immemorial. for help, especially when he struck with ailments. Medicinal plants as a group comprise approximately Undoubtedly the plant kingdom still holds many species 8000 species and account for around 50 % of the highe