Experimental

A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenyl-phosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. Correlations are pre-sented between the manual procedure and those used with both the Technicon Auto-Analyzer and the Robot Chemist. THIS PAPER reports the development of ammautomated procedure for the determination of serum alkaline phosphatase for use ommthe Robot Chemist.t The procedure is essemmtially an adaptation of the Bessey, Lowry and Brock procedure (1) modified by use of a mom-c capable buffer, 2-amino-2-methyl-1-propanol (2). Tn this pi’ocedure, tile p-nitro-phemiol resultimug from the enzyme action is commverted with alkali to the yellow p-nitrophemiolate, whose absorbance is measured at 400 mp.. The report is based ommexperiments conducted with a prototype of time instrument now commercially available