Technetium-99m-Labeled Monoclonal Antibody with Preserved Immunoreactivity and High In Vivo Stability

Recent availability of monoclonal antibodies (MoAb) and their radiolabeling through the use of the bifunctional chelating agents (BCA) have become an alternative procedure for in vivo radioimmunodetection. Using a newly synthesized BCA, a p-carboxyethylphenylglyoxal-di(N-methylthiosemicarbazone) (CE-DTS), the coupling and technetium-99m ("mTc) labeling of monoclonal IgG against hCG were carried out. In the system presented, factors affecting stability and immunoreactivity were examined. Immunoreactivity of the original IgG (56C) was preserved by conjugating one CE-DTS molecule per molecule of IgG (56C) using the phosphorylazide method, however, MmTc labeling pH affected the immunoreactivity and limited the "Te labeling reaction between pH 4.5 and 6.2. A screening of labeling conditions, such as pH, reaction time, and reducing agent system were then carried out. Technetium-99m-labeled IgG (56C), ["TcJCE-DTS-lgG (56C), showed good stability upon incubation with mice sera and comparable mice biodistribution to that of indium-111 (111ln)DTPA-lgG (56C). Thus, these results indicate the excellent potential of CE-DTS as a BCA for labeling MoAb with "Te. J NucÃ-Med 28:1027-1033,198