Impurity Profiling with the Agilent 1200 Series LC System Part 3: Rapid Condition Scouting for Method Development

Method development is one of the most time-consuming tasks in today’s pharmaceutical analytical laboratories.1 Usually, condition scouting is the first step followed by fine-tuning of the best set of parameters found. A means of speeding up the entire process by utilizing Agilent Rapid Resolution HT columns in combination with the Agilent 1200 Series Rapid Resolution LC (RRLC) system is described. How separation condition scouting and method fine-tuning resulted in a robust method for the quantization of diasteromeric and regioisomeric impurities of an active pharmaceutical ingredient within one and a half days is demon-strated. The final method not only is much faster than comparable conventional methods but also saves a significant amount of solvent