Serge X.Cohen, Martine Moulin, Said Hashemolhosseini1, Karin Kilian1,

Glia cell missing (GCM) transcription factors form a small family of transcriptional regulators in meta-zoans. The prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells, whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding, known as the GCM domain. Here we present the crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site at 2.85 AÊ resolution. The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a b-sheet in DNA recognition, whereby a ®ve-stranded b-sheet protrudes into the major groove perpendicular to the DNA axis. The structure com-bined with mutational analysis of the target site and of DNA-contacting residues provides insight into DNA recognition by this new type of Zn-containing DNA-binding domain