Some Biochemical Changes Associated with Injection of Grass Carp (Ctenopharyngodon idellus) with Oviaprim and Pregnyl for Induction of Artificial Spawning

Abstract: Hormonal injection is a common spawning technique in aquaculture season 18 males of grass carp (Ctenopharyngodon idellus) with mean weight of 5 kg and 36 females with mean weight of 8 kg were used. The fish are injected with Ovaprim (Salmon gonadotropin releasing hormone analog, dopamine antagonist and domperidone) and Pregnyl (Human chronic gonadotropin, HCG) (4 mg for female and 2 mg for male /kg BW), 2 injections were given 24hrs a part, either first or second injection. At zero time blood samples were taken from male and females before injection and some biochemical parameters were measured and used as control values. Administration of hormones accelerated the final Oocyte maturation and percent of ovulation. Plasma levels of Estradiol (E2) in female grass carp increased sharply at 6 hrs after the first injection of the two hormones, and it decreased after the second injection. Plasma Testosterone (T) in males increased parallel to the plasma Estradiol in females. Also the results showed significant increase in serum Cortisol, glucose and total protein values during the time of artificial spawning. Key words: Grass carp Spewing hormone Oviaprim Pregnyl INTRODUCTION compared to a lot receiving only saline solution. No Some species of fish will not readily bred in captivity solution), but Estradiol level increases with exogenous due to environmental or culture conditions which may c-GTH, and levels are low at the end of vitellogenesis [4]