© 2003 The British Society for Antimicrobial Chemotherapy A multidrug efflux phenotype mutant of Streptococcus pyogenes

describe a mutant of Streptococcus pyogenes NCTC 8198 with a multidrug efflux phenotype. A mutant selected with ethidium bromide showed a four-fold rise in MIC of norfloxacin, a 16-fold rise in MIC of ethidium bromide and an eight-fold rise in MIC of acriflavine when compared with the parent strain. The MICs were unaffected by the efflux pump inhibitors reserpine, rescinnamine and verapamil. The mutant’s ethidium bromide MIC was reduced two-fold by nor-floxacin. Ethidium bromide accumulation after 10 min was 58 % lower in the mutant compared with the parent. This difference was not affected by carbonyl cyanide m-chlorophenyl-hydrazone