Reports 1283 Absorption Spectra of Corneas in the Far Ultraviolet

Purpose. To study the corneal absorption in the far ultra-violet (UV) region between 260 and 190 nm. Methods. Thirty-four corneal samples of thickness near 20 fj,m were obtained from 18 porcine corneas and six human corneas with a microtome-cryostat. The authors conducted absorbance measurements of the sectioned corneal samples supported by two UV optical windows from 350 to 190 nm using a dual-beam spectrophotome-ter. Three whole porcine corneas were used to study the effect of freezing on the absorbance from 350 to near 290 nm. Results. The absorption spectra of porcine and human corneas from 350 to 190 nm were measured and three segments in the spectrum between 260 and 190 nm have been identified. The linear absorption coefficient