Recombinase-directed transgene placement

The exact placement of foreign DNA into the plant genome produces transgenes with greater structural fidelity and faithful expression. Recombinase-mediated site-specific integration has been reported for several plant species, including rice and maize. The next challenge will be to develop an integrated strategy to stack and translocate DNA. Being able to append new DNA sequentially to a target site permits the continual use of a previously characterized chromosome location, which justifies the initial investment costs in identifying favorable chromosome targets. Stacking transgenic traits at a limited number of target sites is also preferable to scattering transgenes all over the genome, as the clustering of transgenes expedites the introgression of bundled traits to elite cultivars, through a process mediated by the translocation of transgenes from one chromosome to another