Leukocyte Counts in Cerebrospinal Fluid with the Automated Hematology Analyzer CellDyn 3500 and the Urine Flow Cytometer UF-100

cytes in cerebrospinal fluid (CSF) is still performed microscopically, e.g., using a chamber in most laborato-ries. This requires sufficient practical experience, is time-consuming, and may constitute a problem in emer-gency diagnostics. Specific automated systems for CSF cell counting are not available at present. Methods: We tested the hematology analyzer CellDyn 3500 (CD) and the urine flow cytometer UF-100 (UF), which are not designed for CSF analysis. We studied>104 samples with both analyzers, and the counts obtained were compared with the reference method (Fuchs-Rosenthal chamber). Results: Good linearity in the medically relevant range of 15 3 106 to 1000 3 106 leukocytes/L and a high degree of within-run accuracy were seen for both analyzers. Cell counting on the UF was excellent, especially whe