Add to ORKGA high-throughput method for zebrafish sperm cryopreservation Below is a method for zebrafish sperm cryopreservation that is an adaptation of the Harvey method (Harvey et al., 1982). We have introduced two changes to the original protocol that both streamline the procedure and increase sample uniformity. First, we normalize all sperm volumes using freezing media that does not contain the cryoprotectant (i.e. methanol). Second, cryopreserved sperm are stored in cryovials instead of capillary tubes. The rates of sperm freezing and thawing (ƼC/time) are probably the two most critical variable to control in this procedure. For this reason, do not substitute different tubes for those specified. Working in teams of 2 it is possible to freeze the...
Preservation of cells and tissues by freezing at temperatures below 70°C has led to new possibilitie...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the species\u27...
In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow tro...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic...
The synergy obtained by the combination of cryoprotectants is a successful strategy that can be bene...
Cryopreserervation is a powerful tool for the long term storage of genetic material from endangered ...
Initial success in sperm cryopreservation occurred at about the same time for aquatic species and li...
protocol to freeze its spermatozoa to be used in artificial breeding programs aimed at preserving th...
One of the critical points in low temperature storage is the expense of storage capacities. Moreover...
Zebrafish are a highly-valued model organism used for developmental biology research. Zebrafish can ...
For scientific studies on the zebrafish model, simple and routine reproductive procedures should be ...
A new integrated approach including computer-assisted sperm analysis (CASA), viability staining and ...
13 páginas, 2 tablas.Cryopreservation is of interest not only for fish farming but also for the cons...
AbstractThe objective was to develop a suitable freezing method to cryopreserve Brycon opalinus (Cha...
Selection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality i...
Preservation of cells and tissues by freezing at temperatures below 70°C has led to new possibilitie...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the species\u27...
In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow tro...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic...
The synergy obtained by the combination of cryoprotectants is a successful strategy that can be bene...
Cryopreserervation is a powerful tool for the long term storage of genetic material from endangered ...
Initial success in sperm cryopreservation occurred at about the same time for aquatic species and li...
protocol to freeze its spermatozoa to be used in artificial breeding programs aimed at preserving th...
One of the critical points in low temperature storage is the expense of storage capacities. Moreover...
Zebrafish are a highly-valued model organism used for developmental biology research. Zebrafish can ...
For scientific studies on the zebrafish model, simple and routine reproductive procedures should be ...
A new integrated approach including computer-assisted sperm analysis (CASA), viability staining and ...
13 páginas, 2 tablas.Cryopreservation is of interest not only for fish farming but also for the cons...
AbstractThe objective was to develop a suitable freezing method to cryopreserve Brycon opalinus (Cha...
Selection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality i...
Preservation of cells and tissues by freezing at temperatures below 70°C has led to new possibilitie...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the species\u27...
In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow tro...