Tn5 is a composite bacterial transposon that encodes a protein, transposase (Tnp), required for movement of the transposon. The initial step in the transposition pathway involves specific binding of Tnp to 19-bp end recognition sequences. Tn5 contains two different specific end sequences, termed outside end (OE) and inside end (IE). In Escherichia coli, IE is methylated by Dam methylase (IEME). This methylation greatly inhibits recognition by Tnp and greatly reduces the ability of transposase to facilitate movement of IE defined transposons. Through use of a combinatorial random mutagenesis technique (DNA shuffling), we have isolated an IEME-specific hyperactive form of Tnp, Tnp sC7v.2.0, that is able to promote high levels of transposition...
Transposition is the translocation of a DNA fragment, from a donor site to a target site showing no ...
The mobilizable transposon Tn4555 found in Bacteroides spp. is an important antibiotic resistance el...
Transposable elements are an almost ubiquitous feature in all three domains of life, and are a signi...
TnS is a composite transposon consisting of two IS50 sequences in inverted orientation with respect ...
ABSTRACT: X-ray cocrystal structures of Tn5 transposase (Tnp) bound to its 19 base pair (bp) recogni...
The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction d...
Transposons are mobile genetic elements found in all domains of life that are capable of moving betw...
In this study, evidence of novel, important interactions between a hyper-active Tn5 transposon recog...
Transposons are mobile genetic elements that can move between locations in DNA that lack homology. T...
Transposons are mobile genetic elements encountered in all domains of life. They are characterized b...
Transposons are mobile genetic elements that move by transposition from one location to another with...
Transposable elements are an almost ubiquitous feature in all three domains of life, and are a signi...
While non-specific DNA plays a role in target local-ization for many recombinases, transcription fac...
The rate-limiting step in movement of the conjugative transposon Tn916, originally identified in Ent...
Transposons play an integral role in bacterial adaptation, therefore it is important to understand t...
Transposition is the translocation of a DNA fragment, from a donor site to a target site showing no ...
The mobilizable transposon Tn4555 found in Bacteroides spp. is an important antibiotic resistance el...
Transposable elements are an almost ubiquitous feature in all three domains of life, and are a signi...
TnS is a composite transposon consisting of two IS50 sequences in inverted orientation with respect ...
ABSTRACT: X-ray cocrystal structures of Tn5 transposase (Tnp) bound to its 19 base pair (bp) recogni...
The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction d...
Transposons are mobile genetic elements found in all domains of life that are capable of moving betw...
In this study, evidence of novel, important interactions between a hyper-active Tn5 transposon recog...
Transposons are mobile genetic elements that can move between locations in DNA that lack homology. T...
Transposons are mobile genetic elements encountered in all domains of life. They are characterized b...
Transposons are mobile genetic elements that move by transposition from one location to another with...
Transposable elements are an almost ubiquitous feature in all three domains of life, and are a signi...
While non-specific DNA plays a role in target local-ization for many recombinases, transcription fac...
The rate-limiting step in movement of the conjugative transposon Tn916, originally identified in Ent...
Transposons play an integral role in bacterial adaptation, therefore it is important to understand t...
Transposition is the translocation of a DNA fragment, from a donor site to a target site showing no ...
The mobilizable transposon Tn4555 found in Bacteroides spp. is an important antibiotic resistance el...
Transposable elements are an almost ubiquitous feature in all three domains of life, and are a signi...