Mouse Macrophages

All peritoneal macrophage (pM4) populations studied exhibited some binding of the anti-asGM1 serum as assessed by flow cytometry. The levels of reactivity varied quantitatively among populations, depending on the combination of eliciting and activating agents employed prior to the harvest of pM4. Resident pM4i contained a very small percentage (4%) of cells that were strongly asGM1 Any treatment of these cells that induced them to become stimulated or activated increased the percentage of highly asGM1 + cells. Treatments that enhanced anti-asGM1 binding including eliciting pM4 with proteose peptone (16 % asGM1 ) or Brewer’s thioglycollate medium (66 % asGM1 ), treatment with the activating biological response modifiers (BRM5) MVE-2(12 % asGM1 ) and P acnes (18 % asGM1 ), or treatment with both peptone + MVE-2 (37% asGM1 ) or peptone + poly lC/LC (33%). Increased expression of anti-asGM1 was accompanied by some increase in the reactivity of the various pMq populations to treatment with anti-asGM1 serum. This conclusion was base