Divergence between LDL oxidative susceptibility and urinary F2isoprostanes as measures of oxidative stress in type 2 diabetes

sis. Although the most widely used indirect assay to quantify oxidative stress is LDL oxidative susceptibility, direct assays such as urinary F2-isoprostanes have shown great promise. Methods: We evaluated the utility of both a direct measure of oxidative stress (urinary F2-isoprostanes) and an indirect measure of copper-catalyzed, LDL oxi-dation in a model of increased oxidative stress (diabe-tes). We also evaluated an enzyme immunoassay (EIA) method for urinary F2-isoprostanes with a gas chroma-tography–mass spectrometry method. Results: Excellent intraassay and interassay CVs of <4% were obtained with our EIA method. A good correlation was obtained between the two methods (r 5 0.80; n 5 68) of F2-isoprostane measurement. An excellent correlatio