Clone: M21-1721

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide. Description Elk-1 is a 62-kDa member of the Ets oncogene family of transcription factors and a member of the subfamily of ternary complex factors (TCF). Ets proteins mediate a variety of gene activities in response to serum and growth factors. Proteins in the TCF subfamily form a ternary complex by binding to the Serum Response Element (SRE) in conjunction with a dimer of Serum Response Factors (SRF). Elk-1 is phosphorylated by mitogen-activated protein (MAP) kinase pathways in vivo at a cluster of S/T motifs at its carboxy-terminus. Phosphorylation at these sites, particularly at serine 383 (S383), is critical for Elk-1 transcriptional activation. Studies have shown that Elk-1 is a direct target of activated MAP kinase, and that it is also a target of the stress-activated kinase SAPK/JNK. The M21-1721 monoclonal antibody recognizes the phosphorylated S383 of activated Elk-1. The orthologous phosphorylation sites of mouse and rat Elk-1 are S384 and S382, respectively. Analysis Elk-1 (pS383) in activated human peripheral blood mononuclear cells (PBMC). PBMC were isolated by density gradient centrifugation (Ficoll-Paque ™ PLUS, Cat. No. 17-1440-02), and cultured with 20 µg/ml PHA-P (Sigma-Aldrich, Cat. No. L1668) for 3 days, then either lef