Use of Lab-on-a-Chip Technology for Protein Sizing

The performance of the Agilent 2100 bioanalyzer, the first commercial lab-on-a-chip system, and the Protein 200 Plus LabChip ® kit is compared with conventional protein analysis techniques such as SDS-PAGE, Lowry, or Bradford. Lab-on-a-chip technology for protein analysis allows for the inte-gration of electrophoretic separation, staining, destaining, and fluorescence detection into a single process, and for it to be combined with data analysis. The chip-based protein assay allows purity analysis, siz-ing, and relative quantitation based on internal standards or absolute quantitation based on user-defined standards.The chip-based protein analysis is comparable in sensitivity, sizing accuracy, and reproducibility to SDS-PAGE stained with stan-dard Coomassie. Resolution and linear dynamic range are improved.Absolute quantitation accuracy and reproducibil