Urinary proteomics biomarkers in coronary artery disease. Moll. Cell. Proteomics (in press). Biomarker Insights 2008:3

Urinary proteomics is emerging as a powerful non-invasive tool for diagnosis and monitoring of variety of human dis-eases. We tested whether signatures of urinary polypep-tides can contribute to the existing biomarkers for coronary artery disease (CAD). We examined a total of 359 urine samples from 88 patients with severe CAD and 282 con-trols. Spot urine was analyzed using capillary electrophore-sis on-line coupled to ESI-TOF-MS enabling characteriza-tion of more than 1000 polypeptides per sample. In a first step a “training set ” for biomarker definition was created. Multiple biomarker patterns clearly distinguished healthy controls from CAD patients, and we extracted 15 peptides that define a characteristic CAD signature panel. In a sec-ond step, the ability of the CAD-specific panel to predict the presence of CAD was evaluated in a blinded study using