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The key elements concentration, dedication, hard work and application are not the only essential factors for achieving the desired goals but also guidance, assistance and co-operation of people is necessary. I would like to express my deep and sincere gratitude to my supervisor Dr. Anita Rajor Lecturer. Her nice guidance and good suggestion gave me proper way before initiate my thesis work. I would also like to express my deep and sincere gratitude to my supervisor Mr. Somesh Rastogi (Director Effikazy Consulting Pvt. Ltd). His wide knowledge and logical way of thinking have been of great value for me. His understanding and personal soft guidance have provided a good basis for the present thesis. Especially the strict and extensive comments and many discussions and interactions with Mr. Rastogi had a direct impact on the final form and quality of this thesis. I owe my sincere thanks to Dr. N. Das, head of department of biotechnology and Environmental Science for allowed to do the dissertation from out of Thapar campus. I wish to express my sincere thanks for the possible help extended especially by Mr. Somesh Ajnavi, Mr. Rasesh Rastogi and Mr. Mohit Kumar, when ever needed. Without their guidance and suggestion, this work would indeed have been very difficult for me to tackle.I would like to make special acknowledge to some of my colleagues, who have been always there for me, listening to me, rejoicing, complaining and pondering my way throughout my M.Tech., study. I was very fortunate to have unconditional support from my family throughout this time. They have elucidated me the meaning of life, love and living. My loving thanks to my mother who has always kept my morels high through tough time