Selective Potentiation of Lymphocyte-Derived Macrophage Chemotactic Factor Release in Complete Freund’s Adjuvant-Treated Guinea Pigs

Dinitrophenol (DNP)-ovalbumin(OA)-induced tissue macrophage reaction in sensitized guinea pigs is enhanced by treatment with complete Freund’s adjuvant (CFA). The en-hancement of the reaction may be due to the increased production of a T-lymphocyte-derived macrophage chemotactic factor (LDMCF) because treatment of animals with CFA potentiates antigen- and concanavalin A(ConA)-induced release of LDMCF activity from spleen cells of the CFA-treated animals in vitro.This potentiating effect by CFA seems to be ascribed to the release of an adherent-cell-derived soluble factor from the CFA-treated animals. The adherent cell-derived factor, LDMCF-potentiating factor (LDMCF-PF), pref-erentially potentiates the release of LDMCF activity but not of eosinophil chemotactic activity from antigen- or Con-A-stimulated T lymphocytes. Protein synthesis is required for release of LDMCF-PF. Molecular weight of LDMCF-PF activity is assumed to be about 10,000-20,000. LDMCF-PF activity is sensitive to trypsin, to neuraminidase, and also to alkalinity at pH 11, suggesting that LDMCF-PF is a glycoprotein. The present study pro-vides one explanation for the enhanced macrophage reaction in delayed-type hypersen-sitivity reactions. Key words: Lymphocyte-derived eosinophii chemotactic factor (LDECF), LDECF-poten-tiating factor (LDECF-PF), Lymphocyte-activating factor (LAF