Add to ORKGWe can detect the b-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hor-mone gene in the same reaction serves as a control for the amount of template DNA or amplification effi-ciency. Ligation products, which are biotinylated at one end and tagged with an arbitrary “tail ” sequence at the other, are captured by hybridization to “tail”-comple-mentary oligonucleotides immobilized on polystyrene microwells. The captured ligation products are detected colorimetrically by use of streptavidin–alkaline phos-phatase conjugate. In a study of 24 subjects, the assay unequivocally discriminated among normal, carrier, and sickle cell genotypes. INDEXING TERMS: hemo...
Sickle cell disease (SCD) is an inherited red cell disorder, characterized by the tendency of haemog...
BackgroundThe diagnosis of sickle cell disease (SCD) is made by hemoglobin assays such as high-perfo...
Nonisotopic, microwell-based DNA hybridization assays for the specific detection of human immunodefi...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease causing ...
Background – Sickle cell anemia is one of the most common heritable hematologic diseases affecting h...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease-causing ...
DNA diagnostics, the detection of specific DNA sequences, will play an increasingly important role i...
The identification of sporadic point mutations in tumor-suppressor genes, which are responsible for ...
The NucleoLink surface is a physically modified, thermo-stable, optically clear resin. It allows the...
A new nonradioactive oligonucleotide ligation assay for the detection of a common point mutation in ...
Background: Beta-thalassemia is a common autosomal recessive hereditary disease in the Meditertanean...
Sickle Cell Disease (SCD) is a monogenic hereditary blood disorder caused by a single point mutation...
Development of molecular techniques with analytical capability of mutation detection can realize the...
The present thesis work contributes to two areas of molecular genetics, namely the development of me...
A rapid method of gene detection has been developed utilising DNA fragments immobilized on resins an...
Sickle cell disease (SCD) is an inherited red cell disorder, characterized by the tendency of haemog...
BackgroundThe diagnosis of sickle cell disease (SCD) is made by hemoglobin assays such as high-perfo...
Nonisotopic, microwell-based DNA hybridization assays for the specific detection of human immunodefi...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease causing ...
Background – Sickle cell anemia is one of the most common heritable hematologic diseases affecting h...
BACKGROUND: Methods are needed to analyze small amounts of samples for variation in disease-causing ...
DNA diagnostics, the detection of specific DNA sequences, will play an increasingly important role i...
The identification of sporadic point mutations in tumor-suppressor genes, which are responsible for ...
The NucleoLink surface is a physically modified, thermo-stable, optically clear resin. It allows the...
A new nonradioactive oligonucleotide ligation assay for the detection of a common point mutation in ...
Background: Beta-thalassemia is a common autosomal recessive hereditary disease in the Meditertanean...
Sickle Cell Disease (SCD) is a monogenic hereditary blood disorder caused by a single point mutation...
Development of molecular techniques with analytical capability of mutation detection can realize the...
The present thesis work contributes to two areas of molecular genetics, namely the development of me...
A rapid method of gene detection has been developed utilising DNA fragments immobilized on resins an...
Sickle cell disease (SCD) is an inherited red cell disorder, characterized by the tendency of haemog...
BackgroundThe diagnosis of sickle cell disease (SCD) is made by hemoglobin assays such as high-perfo...
Nonisotopic, microwell-based DNA hybridization assays for the specific detection of human immunodefi...