Monoclonal Antibody-Based Time-Resolved Fluorescence Immunoassays for Daidzein, Genistein, and Equol in Blood and Urine: Application to the Isoheart Intervention Study

says (TR-FIAs) for phytoestrogens in biological samples are an alternative to mass spectrometric methods. These immunoassays were used to test urine and plasma samples from individuals in a dietary intervention trial aimed at determining the efficacy of dietary isoflavones in reducing the risk of coronary heart disease in post-menopausal women. Methods: We established murine monoclonal TR-FIA methods for daidzein, genistein, and equol. These as-says could be performed manually or adapted to an automated analyzer for high throughput and increased accuracy. Analysis of urine was conducted on nonex-tracted samples. Blood analysis was performed on non-extracted samples for daidzein, whereas genistein and equol required diethyl-ether extraction