Supplemental Experimental Procedures

pCMV-myc-avrA and pCMV-myc-mavrA are described previously (Collier-Hyams et al., 2002). The mAvrA mutant is a catalytically inactive form of AvrA, in which the cysteine residue at position 186 is substituted to an alanine. A plasmid harboring the aopP coding sequence was a gift from Joachim Frey. A catalytically inactive form of AopP (mAopP) was created by overlap extension PCR where the cysteine residue at position 177 was substituted to an alanine. The aopP and maopP coding sequences were cloned into pCMV-myc, creating pCMV-myc-aopP and pCMV-myc-maopP respectively. Plasmids harboring vopA or mvopA coding sequences were a gift from Kim Orth. The vopA and mvopA coding sequences were cloned into pCMV-myc, creating pCMV-myc-vopA and pCMV-myc-mvopA respectively. pcDNAflag-JNK1 and pcDNAflag-MKK7 were a gift from Prof. Roger Davis. The JNK1 coding sequence was cloned into pCMV-myc to facilitate simultaneous detection of JNK1 and MKK7 expression in lysates of transfected cells. DNA amplicons of myc-avrA, myc-mavrA, myc-aopP, myc-maopP, myc-vopA or myc-mvopA were cloned into pP[UAST] (a gift from Kevin Moses) creating pP[UAST]-myc-avrA, pP[UAST]-myc-mavrA, pP[UAST]-myc-aopP, pP[UAST]-myc-maopP, pP[UAST]-myc-vopA or pP[UAST]-myc-mvopA respectively. Drosophila lines. The vectors pP[UAST]-myc-avrA, pP[UAST]-myc-mavrA, pP[UAST]-myc-aopP