Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily

ABSTRACT: The amidohydrolase superfamily comprises hundreds of hydrolytic enzymes of the (â/R)8 barrel fold with mono- or binuclear active-site metal centers, and a diverse spectrum of substrates and reactions. Promiscuous activities, or cross-reactivities, between different members of the same superfamily may provide important hints regarding evolutionary and mechanistic relationships. We examined thre