Trichophyton species: use of volatile fingerprints for rapid identification and discrimination

Background: Fungal infection of the skin is a common clinical problem, and laboratory confirmation of the diagnosis is important to ensure appropriate treatment. The identification of the species of fungus is also important, because different fungal species have different modes of transmission, and this may be of importance both in preventing re-infection or in avoidance of infection of others. Objective: This study examined the potential of using volatile production patterns for the detection and discrimination between four Trichophyton species (T. mentagrophytes, T. rubrum, T. verrucosum and T. violaceum) in vitro on solid media and in broth culture. Methods: Two different sensor array systems (conducting polymer and metal oxide sensors) were examined for comparing the qualitative volatile fingerprints produced by these species over periods of 24-120 hrs in the headspace. The relative sensitivity of detection of two of the species (T. mentagrophytes, T. rubrum) was determined for log1 to log7 inoculum levels over the same time period. Results: The conducting polymer based system was unable to differentiate between species based on volatile fingerprints over the experimental period. However, metal oxide-base