Four Loops of the Catalytic Domain of Factor VIIa Mediate the Effect of the First EGF-like Domain Substitution on Factor VIIa Catalytic Activity

The presence of tissue its full catalytic poten Blood coagulation factor VIIa (FVIIa) is a tryp-E-mail address of the corresponding author: doi:10.1006/jmbi.2001.4556 available online at http://www.idealibrary.com on J. Mol. Biol. (2001) 307, 1503–1517sin-like plasma serine protease. FVIIa in its cataly-lee_pedersen@unc.edu Abbreviations used: EGF1, the first epidermal growth factor-like domain; EGF2, the second epidermal growth factor-like domain; FVII, factor VII; FVIIa, activated factor VII; FVII(IXegf1), FVII with its EGF1 domain substituted by that of FIX; FVII(IXegf1)a, activated FVII (IXegf1); FIX, factor IX; FX, factor X; RMSD, root-mean-square deviation; SAA, solvent accessible area; Gla, g-carboxyglutamic acid; TF, tissue factor; TF-f1, residues 6 to 80; TF-f2, residues 91 t