Rate of Decrease of Glutamyltransferase and Acid Phosphatase Activities in the Human Vagina after Coitus

I studied the rate at which gamma-glutamyltransferase (EC 2.3.2.2) and acid phosphatase (EC 3.1.3.2) activities de-crease in the healthy human vagina after coitus. Speci-mens from the coital and non-coital vagina, and from the rectum and buccal cavity, and pre-ejaculate lubricating fluid were also assayed for both enzymes. Prostatic acid phosphatase was determined with use of two substrates: thymolphthalein monophosphate and a-naphthyl phos-phate (tartrate-inhibitable fraction). Glutamyltransferase was assayed with ‘y-glutamyl-p-nitroanilide as substrate. All non-coital specimens were negative for glutamyl-transferase activity; the activity of acid phosphatase varied with type of substrate and type of specimen. The equation y = a + (b/x) describes the decline in activity (y) of either acid phosphatase or glutamyltransferase in the vagina o