Supplemental Experimental Procedures

All strains were maintained at 15°C using standard C. elegans techniques (Stiernagle, 2006). Double mutants were made using standard genetic methods as described below. For all RNAi assays, the worms were grown for at least two full generations on the RNAi bacteria. For making double mutants, daf-2(e1370) males were mated to either akt-1(ok525), akt-2(ok393) and sgk-1(ok538) hermaphrodites, respectively. A total of 40 putative F1 cross progeny were singled onto individual plates and allowed to have progeny at 25°C. The F2 dauers were then selected and allowed to recover at 15°C. The recovered dauers were singled and transferred to 25°C and allowed to have progeny. For plates where the F3 progeny formed 100 % dauers at 25°C, parents were tested for akt-1(ok525), akt-2(ok393) or sgk-1(ok538) deletion by PCR. The primers used for the PCR analysis are listed in Supplemental Table 6. For making the daf-2(e1370); daf-3(mgDf90) double mutant, daf-2(e1370) males were crossed to daf-3(mgDf90) hermaphrodites. The F1 progeny males (daf-2(e1370)/+; daf-3(mgDf90)) were selected and mated back to daf-3(mgDf90) hermaphrodites. Forty putative F2 cross progeny were transferred to individual plates and incubated at 25°C. After 4-5 days, parents were selected from plates where the F3 progeny were 100 % dauers and the daf-