APPLICATION OF COUNTERCURRENT CHROMATOGRAPHY

One of the few liquid chromatographic techniques that can be predictably scaled up from analytical to process scale is countercurrent chromatography (CCC)1,2. In combination with the 100 % recovery of the sample and the gentle separation conditions, this technique is ideally suited to the analysis of polar wine constituents. In the first part of the lecture, CCC instrumentation and its application to the analysis of, e.g., labile aroma precursors3, antioxidants4,5 and anthocyanins6,7 will be presented. In the second part, novel CPC instrumentation8 for the fractionation of polymeric wine constituents as well as the scale-up of the technique9,10 for separations in the 10-100 g range will be described