Protein Profiling of Microdissected Pancreas Carcinoma and Identification of HSP27 as a Potential Serum Marker

have a poor prognosis because of late clinical manifes-tation and the tumor’s aggressive nature. We used pro-teomic techniques to search for markers of pancreatic carcinoma. Methods: We performed protein profiling of microdis-sected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using Protein-Chip technology (surface-enhanced laser desorption/ ionization). We identified proteins by use of 2-dimen-sional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals