DYX1C1 functions in neuronal migration in developing neocortex. Neuroscience 143

Abstract—Rodent homologues of two candidate dyslexia susceptibility genes, Kiaa0319 and Dcdc2, have been shown to play roles in neuronal migration in developing cerebral neocortex. This functional role is consistent with the hypoth-esis that dyslexia susceptibility is increased by interference with normal neural development. In this study we report that in utero RNA interference against the rat homolog of another candidate dyslexia susceptibility gene, DYX1C1, disrupts neuronal migration in developing neocortex. The disruption of migration can be rescued by concurrent overexpression of DYX1C1, indicating that the impairment is not due to off-target effects. Transfection of C- and N-terminal truncations of DYX1C1 shows that the C-terminal TPR domains determine DYX1C1 intracellular localization to cytoplasm and nucleus. RNAi rescue experiments using truncated versions of DYX1C1 further indicate that the C-terminus of DYX1C1 is necessary and sufficient to DYX1C1’s function in migration. In conclusion, DYX1C1, similar to two other candidate dys-lexia susceptibility genes, functions in neuronal migration in rat neocortex. © 2006 IBRO. Published by Elsevier Ltd. All rights reserved. Developmental dyslexia is associated with both genetic susceptibility (Pennington and Smith, 1983; Smith et al., 1998) and migration anomalies in neocortex (Galaburd