Add to ORKGAlmost all chemical reactions and transport processes in a cell are catalysed by specific enzymes and transport proteins, respectively. Kinetic characterization of these auxiliary proteins is a necessary prerequisite for understanding the dynamics and regulation of cellular reactions networks. Progress-curve analysis, i.e. estimation of kinetic parameters by fitting of integrated rate laws to the time-course of a biochemical reaction, allows an efficient kinetic characterization of enzymes. This article outlines the mathematical fundamentals of progress-curve analysis and provides examples for the application of this method in enzyme kinetics and system biology
The enzyme catalyzed hydrolysis of para-nitrophenylphosphate was studied using progress curve analys...
A new method is described for estimating initial velocities of enzyme-catalysed reactions. It is sim...
Assuming the in vitro conditions for the enzyme-catalyzed reactions, the basic Michaelis-Menten desc...
Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes di...
There are several advantages to the use of progress curves to analyze the kinetic properties of enzy...
With computer-based data-fitting methods becoming a standard tool in biochemistry, progress curve an...
With computer-based data-fitting methods becoming a standard tool in biochemistry, progress curve an...
The computer analysis of progress curves for enzyme-catalysed reactions involves a series of mathema...
Most methods for studying the kinetic properties of an enzyme involve the determination of initial v...
In a coupled system of two enzyme-catalysed reactions the concentration of the final product follows...
The aim of the research is to analize and model the progress of product accumulation in relation wit...
which permits unrestricted use, distribution, and reproduction in any medium, provided the original ...
Mathematical modelling of metabolic pathway provides us a wide variety of information about behavio...
[eng] The evaluation of individual rate constants involved in any reaction mechanism of an enzymatic...
Enzymes, like all positive catalysts, dramatically increase the rate of a given reaction. Enzyme kin...
The enzyme catalyzed hydrolysis of para-nitrophenylphosphate was studied using progress curve analys...
A new method is described for estimating initial velocities of enzyme-catalysed reactions. It is sim...
Assuming the in vitro conditions for the enzyme-catalyzed reactions, the basic Michaelis-Menten desc...
Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes di...
There are several advantages to the use of progress curves to analyze the kinetic properties of enzy...
With computer-based data-fitting methods becoming a standard tool in biochemistry, progress curve an...
With computer-based data-fitting methods becoming a standard tool in biochemistry, progress curve an...
The computer analysis of progress curves for enzyme-catalysed reactions involves a series of mathema...
Most methods for studying the kinetic properties of an enzyme involve the determination of initial v...
In a coupled system of two enzyme-catalysed reactions the concentration of the final product follows...
The aim of the research is to analize and model the progress of product accumulation in relation wit...
which permits unrestricted use, distribution, and reproduction in any medium, provided the original ...
Mathematical modelling of metabolic pathway provides us a wide variety of information about behavio...
[eng] The evaluation of individual rate constants involved in any reaction mechanism of an enzymatic...
Enzymes, like all positive catalysts, dramatically increase the rate of a given reaction. Enzyme kin...
The enzyme catalyzed hydrolysis of para-nitrophenylphosphate was studied using progress curve analys...
A new method is described for estimating initial velocities of enzyme-catalysed reactions. It is sim...
Assuming the in vitro conditions for the enzyme-catalyzed reactions, the basic Michaelis-Menten desc...