Original Article UNCONFIRMED REACTIVE SCREENING TESTS AND THEIR IMPACT ON DONOR MANAGEMENT

Objective: To determine the percentage of false positive testing for transfusion transmitted infections (TTIs) using immunochromatographic test (ICT) as first line of screening tests and its effect on loss of volunteer blood donors. Methodology: Over a period of three months, samples from blood bags of donors undergoing phlebotomy at teaching hospital blood banks in Lahore were screened for human immunodeficiency virus (HIV), hepatitis B (HBV) and hepatitis C (HCV) by immunochromatographic tests. Those found positive on initial screening were re-tested by ELISA method at the screening laboratory of the Institute of Haematology & Blood Transfusion Service, Punjab. Lahore. Results: Out of a total of 62090 voluntary blood donors, 469 donors were found to be initially reactive for either HIV, HBV or HCV. Amongst these 96(0.15%) blood donors were found to have tested falsely positive for HIV, HBV or HCV as compared to testing by ELISA. Conclusions: False positive testing rate of 0.15 % or 96 out of a total of 62090 donors is rather small in terms of loss of voluntary donors and appropriate utilization of available resources. Although immunochromatographic testing is not the gold standard, however it serves an important purpose of initial donor screening. KEY WORDS: ICT & TTIs, FP testing, Loss of volunteer blood donors. Pak J Med Sci July- September 2008 Vol. 24 No. 4 517-519 How to cite this article: Rahman M, Khan SA, Lodhi Y. Unconfirmed reactive screening tests and their impact on dono