Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue of mammalian soluble adenylyl cyclase. sGC provides nearly all chemoattractant-stimulated cGMP formation and is essential for efficient chemotaxis toward cAMP. We show that in resting cells the major fraction of the sGC-GFP fusion protein localizes to the cytosol, and a small fraction is associated to the cell cortex. With the artificial substrate Mn2/GTP, sGC activity and protein exhibit a similar distribution between soluble and particulate fraction of cell lysates. However, with the physiological substrate Mg2/GTP, sGC in the cytosol is nearly inactive, whereas the particulate enzyme shows high enzyme activity. Reconstitution experiments re...
The chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several second mes...
The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer ...
Aggregating Dictyostelium cells secrete cAMP during cell aggregation, cAMP induces two fast response...
Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue o...
Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue o...
Chemotaxis of amoeboid cells is driven by actin filaments in leading pseudopodia and actin–myosin fi...
Chemotaxis of amoeboid cells is driven by actin filaments in leading pseudopodia and actin–myosin fi...
Guanylyl cyclase A (GCA) and soluble guanylyl cyclase (sGC) encode GCs in Dictyostelium and have a t...
Guanylyl cyclase A (GCA) and soluble guanylyl cyclase (sGC) encode GCs in Dictyostelium and have a t...
Chemoattractants transiently activate guanylyl cy-clase in Dictyostelium discoideum cells. Mutant an...
AbstractThe chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several se...
Chemoattractants transiently activate guanylyl cyclase in Dictyostelium discoideum cells. Mutant ana...
The chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several second mes...
The chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several second mes...
The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer ...
Aggregating Dictyostelium cells secrete cAMP during cell aggregation, cAMP induces two fast response...
Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue o...
Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue o...
Chemotaxis of amoeboid cells is driven by actin filaments in leading pseudopodia and actin–myosin fi...
Chemotaxis of amoeboid cells is driven by actin filaments in leading pseudopodia and actin–myosin fi...
Guanylyl cyclase A (GCA) and soluble guanylyl cyclase (sGC) encode GCs in Dictyostelium and have a t...
Guanylyl cyclase A (GCA) and soluble guanylyl cyclase (sGC) encode GCs in Dictyostelium and have a t...
Chemoattractants transiently activate guanylyl cy-clase in Dictyostelium discoideum cells. Mutant an...
AbstractThe chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several se...
Chemoattractants transiently activate guanylyl cyclase in Dictyostelium discoideum cells. Mutant ana...
The chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several second mes...
The chemoattractant cAMP induces directed cell locomotion in Dictyostelium cells. Several second mes...
The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer ...
Aggregating Dictyostelium cells secrete cAMP during cell aggregation, cAMP induces two fast response...