Gas-ChromatographicIdentification of Urinary Carbohydrates Isolated by Anion-ExchangeChromatography

More than 40 chromatographic peaks representing carbohydrates have been separated from human urine by a carbohydrate analyzer that makes use of anion-exchange chromatography. Ten of these separated carbo-hydrates have been identified by gas chromatography of their trimethyl-silyl derivatives, which were formed by reaction with N-trimethylsilylimid-azole. The borate buffer from the anion-exchange separation was removed from the samples before the derivatives were made. Gas-chromatographic retention data have been determined for 37 carbohydrates, including pen-toses, hexoses, hexosamines, disaccharides, and trisaccharides, and are tabulated as methylene unit (MU) values. These various classes of carbo-hydrates elute within distinct MU regions, generally in the order of increas-ing molecular weight. More than one peak is obtained for those sugars existing in more than one anomeric form. Additional Keyphrases trimethylsilyl derivatives #{149}“methylene unit ” values molecular weight-elution relationship