A complex-centric view of protein network evolution. Nucleic Acids Res

The recent availability of protein–protein interaction networks for several species makes it possible to study protein complexes in an evolutionary context. In this article, we present a novel network-based framework for reconstructing the evolutionary his-tory of protein complexes. Our analysis is based on generalizing evolutionary measures for single proteins to the level of whole subnetworks, compre-hensively considering a broad set of computation-ally derived complexes and accounting for both sequence and interaction changes. Specifically, we compute sets of orthologous complexes across species, and use these to derive evolutionary rate and age measures for protein complexes. We observe significant correlations between the evolutionary properties of a complex and those of its member proteins, suggesting that protein complexes form early in evolution and evolve as coherent units. Additionally, our approach enables us to directly quantify the extent to which gene duplication has played a role in the evolution of complexes. We find that about one quarter of the sets of orthologous complexes have originated from evolutionary cores of homodimers that under-went duplication and divergence, testifying to the important role of gene duplication in protein com-plex evolution