Genetic analysis in some Citrus accessions using

Twelve ISSR primers, five SSR primer pairs and five AFLP primer combinations were used to estimate the level of polymorphism among 14 Citrus genotypes. The two types of ISSR primers (unanchored and anchored) showed different levels of polymorphism. All of the unanchored primers successfully amplified bands with a total number of 54 of which 44 were polymorphic, representing a percentage of polymorphism of 79.14 % and 8.8 polymorphic bands per primer. While, three out of the four 5’-anchored primers amplified scorable bands revealing a total number of 34 bands of which 28 were polymorphic, corresponding to 82.4 % polymorphism and an average of 9.3 polymorphic bands per primer. On the other hand, the 3’-anchored primers failed to amplify reproducible patterns even with the modification of the PCR-reaction and temperature profile. Four sets out of five SSR primer pairs amplified a total number of 23 alleles revealing 100% polymorphism, with an average of 5.8 polymorphic alleles per loci. The SSR markers revealed allele frequency ranging from 0.04 to 0.52. The expected heterozygosity ranged from 0.65 to 0.83 and the effective alleles per locus ranged from 2.9 to 5.9. The five AFLP primer combinations amplified a total number of 311 amplicons, where 304 were polymorphic representing 97.7