Modification of Platelet Shape Change Parameter by Modified Phospholipids in Oxidized LDL

Background: It is believed that the oxidatively modified lipoproteins play a critical role in activating platelets and is a contributing factor in the etiology of a number of cardiovascular-related diseases. Objective: Identify the active component(s) of oxidized LDL that initiated shape change in plasma-free human platelets prepared by a gel filtration method. Material and Method: Shape change parameter of platelets was monitored following exposure platelets to LDL, copper sulfate-oxidized LDL, and different types of lipids extracted of the corresponding LDL. Results: Oxidized LDL, but not native LDL, increased the shape-change parameter in a concentration-depen-dent manner. Specifically, phosphatidyl serine from oxidized LDL was responsible for this effect. Conclusion: Oxidized phospholipids generated during the oxidative modification of LDL are likely to be the active components responsible for changes in platelet function