Continuous-FlowZone Electrophoresis:ExperimentalDemonstrationand

We report continuous-flow sample application and con-tinuous electrophoretic separation on a moving cellulose acetate tape. This very simple automated electrophoretic system requires minimum electronic control circuitry. Samples and calibrators are pumped by a peristaltic pump from sample containers onto a continuously moving cel-lulose acetate tape. Applied samples are interspaced with rinse solution (buffer) and small air segments for sample integrity. Electrophoretic separation, staining, and de-staining are all continuous functions performed on the continuously moving tape. The system appears suitable for separation of proteins. Continuous-flow application could be combined with discrete sample application onto the same tape for continuous counter-immunoelectro-phoresis; moreover, counter-electrophoresis of tw