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Objective: Personality psychology has for many years been looking for a taxonomy permitting the study of specified personality characteristics. The Big Five structure captures the common existing systems of personality description, and provides an integrative descriptive model for personality research. The way people are behaving when using the Internet, the Internet motives they have, and the choices they are making then, is very much de-termined by their individual characteristics. The purpose of the study was to investigate the relationship between the motives for Internet uses and the Big Five personality characteristics. Method: 300 medical students filled out questionnaires connected to Internet motives, Internet affinity and the brief 44-items BFI (the Big Five Inventory; John, 1990). The sample consisted of 150 Hungarian (75 males and 75 females) and 150 Israeli (75 males and 75 females) medical students. Results: There were significant gender differences in the Israeli sample between the Big Five and Internet motives. The results showed that the Israeli males and females were both high on agreeableness and the Internet motive of habit. Furthermore, the Israeli students scoring high on the personality factor of neuroticism also scored high on the Internet motive of companionship. When it came to the Hungarian sample, students scored highest on conscientiousness, but the males and females scored high on different Internet motives. This means that conscientious Hungarian males obtained higher scores for the companionship motive, while the conscientious Hungarian females scored high on the Internet motive of pass time. Internet affinity significantly correlated with extraversion and neuroticism for the Israeli sample but not for the Hungarian sample. Our results suggest that personality characteristics have important effects on Internet uses.