Four new Type I restriction enzymes identified in Escherichia coli clinical isolates

Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschi-zomers (EcoBI and Eco377I) were identified in a col-lection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)